Background: Chronic Obstructive Pulmonary Disease (COPD) would be the third leading cause of death by 2030 as per WHO. India has close to 30 million COPD patients at present. Acute exacerbations in these patients are associated with worse outcomes and are responsible for a decline in lung function. Bacterial infections cause exacerbations in 30-50% cases and new bacterial strains are often isolated; they are different in India than what Western studies report. Hence, it is useful to know the bacteriological profile of the prevalent strains as per geography so as to manage the exacerbations better. Aim and Objectives: The study aimed to determine the bacteriological profile and sensitivity to antibiotics in sputum of patients with Acute Exacerbation of COPD (AECOPD). Material and Methods: Ninety patients presenting with Acute Exacerbation of Chronic Obstructive Pulmonary Disease (AECOPD) who met the inclusion criteria were included and their sputum was sent for Gram stain, Ziel-Neelson stain and bacterial culture and antibiotic sensitivity testing. Results: Culture was positive for pathogens in 34 (37.7%) patients. P. aeruginosa 14 (41.1%), K. pneumoniae 11 (32.35%) and E. coli 9 (26.47%) were the organisms isolated. The isolates were sensitive to Beta-Lactam/Beta-Lactamase Inhibitor (BL/BLI) combinations, carbapenems and colistin and polymyxin B. P. aeruginosa and E. coli were also sensitive to aminoglycosides and fluroquinolones while K. pneumoniae was variably resistant to them. Age, gender and leucocyte count were not significantly associated with the sputum positivity rate. Conclusion: Cefoperazone/sulbactam with an aminoglycoside and/or levofloxacin may be considered for initiation of therapy in AECOPD patients.