The objective of this study was to ascertain sperm population and cellular characteristics as well as total antioxidant capacity in spermatozoa from Holstein bulls with Good (11 bulls) and Poor (5 bulls) cryotolerance. Post-thaw sperm kinetics were evaluated using CASA, membrane integrity was assessed via HOS test, and DNA fragmentation was measured using the HaloSperm kit. Data were analyzed using principal component analysis. The spermatozoa from Good bulls had a higher number of cells with intact membranes (P=0.029), non-fragmented DNA (P=0.018), and post-thaw viability (P