将亮斑扁角水虻幼虫(black soldier fly larvae,BSFL)接入含禽腺病毒血清4型(FAdV-4)的鸡粪中(0~18 d),采用TaqMan qPCR和16S rDNA高通量测序分析了 BSFL转化过程中FAdV-4含量的动态变化和微生物区系演化.结果表明,BSFL转化能够显著消减鸡粪中的FAdV-4群体载量,转化18 d时FAdV-4减少率达到了 99.63%,明显高于对照组(64.74%),并且增加BSFL接种量和日龄能增强对FAdV-4的消减作用;BSFL转化使得物料中的细菌多样性显著降低;厚壁菌门、假单胞菌属受到抑制,而拟杆菌门、异常球菌-栖热菌门丰度则明显提高;居绿藻菌属、特吕珀菌属在BSFL转化体系中与FAdV-4消减密切相关.
Black soldier fly larvae(BSFL)were introduced into chicken manure containing fowl adenovirus serotype 4(FAdV-4)for 0-18 d.We analyzed the dynamic changes of FAdV-4 content and microflora evolution during BSFL transformation by TaqMan qPCR and 16S rDNA high-throughput sequencing.The results show that BSFL transformation can significantly reduce the mass load of FAdV-4 in chicken manure,and the reduc-tion rate of FAdV-4 is 99.63%after transformation for 18 d,which is significantly higher than that of the con-trol group(64.74%);meanwhile,increasing the dose and age of BSFL can enhance the reduction effect.The BSFL transformation can significantly reduce the diversity of bacteria in the materials.Firmicutes and Pseudo-monas are suppressed,while the relative abundances of Bacteroidota and Deinococcus-Thermus are significantly increased.Ulvibacter and Truepera are closely related to FAdV-4 reduction in BSFL transformation system.