目的 观察脑死亡对猪肝脏免疫原性的影响.方法 健康猪12头,随机分为2组:对照组6头,仅行麻醉维持24h;脑死亡组6头,建立猪脑死亡模型并维持脑死亡状态24h.脑死亡后(对照组持续麻醉后)6、12、24h取血标本及开腹取同一部位肝组织,利用ELISA法检测血清肿瘤坏死因子α(TNFα)、白细胞介素-6(IL-6)水平变化,实时荧光定量PCR法观察肝组织细胞间黏附分子-1(ICAM-1)、单核细胞趋化蛋白-1(MCP-1)mRNA变化,免疫组织化学法观察肝组织主要组织相容性复合体II(MHC-II)表达变化.结果 各时间点脑死亡组血清TNFα、IL-6水平、肝组织ICAM-1、MCP-1mRNA转录水平及肝组织MHC-II表达水平均较对照组高,两者相比差异有统计学意义(P<0.05).结论 脑死亡状态增加了肝组织的免疫原性.可能潜在增加了移植后供肝的免疫损伤及排斥反应.
Objective To observe the effect of brain-dead state on hepatic immunogenicity of pigs. Methods Twelve healthy pigs were randomized into a control group with maintenance of anesthesia alone for 24 h and a brain-death group with maintenance of brain-dead state for 24 h, 6 pigs each. Serum samples and hepatic tissues in the same locus were taken at 6 h, 12 h, and 24 h after anesthesia or the establishment of brain death. The ELISA method was used to determine tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6); the real-time RT-PCR was used to observe the changes of ICAM-1 and MCP-1 mRNA; the immunity tissues chemistry method was used to evaluate the expression of MHC-II. Results The serum levels of TNF-α and IL-6, the transcription levels of ICAM-1 and MCP-1mRNA, and the expression of MHC-II in hepatic tissues in the brain-dead group were higher than those in the anesthesia group, with a statistical significance(P<0.05). Conclusion The brain-dead state increases the immunogenicity of liver tissues, which may aggrevate the immune lesion and increase the risk of reject reaction after transplantation.