在研究乳酸克鲁维酵母(Kluyveromyces lactics)生长特性基础上,确定了该菌株最佳碳源和用量为蔗糖23.8 g/L,最佳氮源和用量为酵母浸粉10.0 g/L;研究发现,与静息细胞催化相比,乳酸克鲁维酵母(Kluyveromyces lactics)在培养 18 h后添加底物苯乙酮催化更加有利于转化为(R)-1-苯乙醇;最后对苯乙酮催化工艺进行了5因素4水平的正交实验优化.结果表明,Kluyveromyces lactics 细胞采用蔗糖23.0g/L,酵母浸粉 10.0g/L,磷酸二氢钾 2.0g/L,七水合硫酸镁 1.5g/L, 30℃条件下培养18 h后,每隔4 h分3次添加底物苯乙酮,底物容量为249 mmol/L,整细胞催化24 h,(R)-1-苯乙醇化学收率95%,光学收率均达到99.5%以上.
The optimal carbon and nitrogen sources and usages were determined on the base of the research of the Kluyveromyces lactics growth,respectively with the sucrose 23.8 g/L and the yeast extract powder 10. 0 g/L.The Kluyveromyces lactics cell after culturing 1 8 hrs was better for Acetophenone to (R)-1-phenyl ethanol compared with the resting cell.Finally,the orthogonal test with L16(45)was performed for the opti-mization of the biocatalytical process with the whole cell.The results show that the chemical yield of the ace-tophenone to(R)-1-phenyl ethanol can be up to 95%,the optical yield of(R)-1-phenyl ethanol can be up to 99.5% after 24 hrs of the bioconversion with the substrate concentration of the acetophenone 249 mmol/L by three times addition when the Kluyveromyces lactics cell was cultured 18hrs under the conditions of the su-crose23.0 g/L and the yeast extract powder 10.0 g/L K2HPO42.0g/L,MgSO4.7H2O 1.5g/L at 30℃.