目的 观察实验性牙周炎对小鼠焦虑样行为的影响,初步探讨肠道菌群在其中发挥的作用.方法 16 只雄性C57BL/6 小鼠随机分为两组:对照组和牙周炎组,牙周炎组采用丝线诱导实验性牙周炎.8 周后采用旷场实验和高架十字迷宫实验检测小鼠焦虑样行为;使用 16S rRNA测序检测两组小鼠粪便肠道菌群变化.结果 与对照组相比,牙周炎组小鼠牙槽骨吸收,在旷场实验中进入中心区域时间减少,高架十字迷宫实验中进入开臂时间减少.16S rRNA测序发现两组小鼠肠道菌群β多样性存在统计学差异,牙周炎组厚壁菌门/拟杆菌门比值显著下降.LEfSe分析结果显示,对照组差异菌为Acetatifactor,Oscillospirales,Coriobacteriia,Butyricicoccaceae,Coriobacteriales,牙周炎组差异菌为Peptococcus,Bacteroidia,Bacteroidota,Muribaculaceae,Bacteroidales.相关性分析结果显示,norank_f__Muribaculaceae与高架开臂时间呈负相关,Akkermansia与高架开臂时间呈正相关.功能预测分析提示两组肠道菌群脂肪酸降解通路存在差异.结论 实验性牙周炎诱导小鼠产生焦虑样行为,肠道菌群结构功能变化可能发挥一定作用.
Objective To observe the effect of experimental periodontitis on anxiety-like behavior in mice,and to initially explore the role played by gut microbiota.Methods Sixteen male C57BL/6 mice were randomly divided into control group and periodontitis group.The periodontitis group was treated with ligature-induced experimental periodontitis.After 8 weeks,open field test and elevated plus maze test were used to detect the anxiety-like behavior of mice.16S rRNA sequencing was used to detect the changes of gut microbiota in the feces of the two groups of mice.Results Compared with the control group,the periodontitis group showed alveolar bone resorption,reduced central zone time in the open field test,and reduced open arms time in the elevated plus maze test.16S rRNA sequencing revealed a significant difference in the β-diversity of gut microbiota between the two groups.The ratio of Firmicutes to Bacteroidetes was significantly decreased in the periodontitis group.LEfSe analysis showed that Acetatifactor,Oscillospirales,Coriobacteriia,Butyricicoccaceae,Coriobacteriales in the control group and Peptococcus,Bacteroidia,Bacteroidota,Muribaculaceae,Bacteroidales in the periodontitis group had significant difference on sample classification.The results of correlation analysis showed that norank_f__Muribaculaceae was negatively correlated with the open-arm time,and Akkermansia was positively correlated with the open-arm time.Functional prediction analysis suggested that there were differences in the fatty acid degradation pathways of gut microbiota between the two groups.Conclusion Experimental periodontitis induces anxiety-like behavior in mice,and changes in the structure and function of gut microbiota may play a role.