背景:目前骨组织工程中,支架材料的生物相容性和生物力学性能都不尽满意,后续的材料降解也是亟待解决的问题.新材料、方法、技术以及医学研究相结合,可以使骨组织工程中支架的外形与结构、生物相容性、降解速率都有很好的改进.目的:通过介孔纳米羟基磷灰石/聚乳酸(nano-hydroxyapatite/poly lactic acid,nHA/PLLA)复合物的支架构建以及与成骨细胞的相容性研究,旨在开发由2种材料复合而成具有更好的生物相容性的骨组织工程支架.方法:采取冷冻快速成型法制备nHA/PLLA复合物,BET法测定孔隙率,扫描电镜(scanning electron miscroscopy,SEM)观察材料内部结构等进行物理性能表征,X线衍射(X-ray diffraction,XRD)表征材料物理学特性.SEM观察成骨细胞在nHA/PLLA内生长、黏附、增殖情况;荧光倒置显微镜观察并计算细胞在该支架材料内的存活情况;MTT实验测定nHA/PLLA复合物对成骨细胞的活性影响.结果:制备的nHA/PLLA复合物,通道孔径为(164±52)μm,介孔率为89.3%±1.4%;干性和湿性nHA/PLLA复合支架的压缩模量分别为(1354.6±53.7)kPa和(1012.8±61.3)kPa.MTT实验结果表明,nHA/PLLA复合物对成骨细胞活性无显著影响,随着孵育时间的延长,黏附在复合物表面及介孔中的细胞数量逐渐增加.结论:nHA-PLLA复合物是一种性能良好的骨组织工程支架材料,具有制备简单、外形及内部结构符合组织工程支架要求、与成骨细胞的生物相容性好等优点.
Background:At present,the biocompatibility and biomechanical properties of scaffold materials are not satisfactory in bone tissue engineering,and the subsequent degradation of materials is also a problem to be solved.The combination of new materials,methods,techniques and medical research could improve the shape and structure,biocompatibility and degradation rate of scaffolds in bone tissue engineering.Objective:To develop a compound of two materials with better biocompatibility in bone tissue engineering through the construction of scaffolds by mesoporous nano hydroxyapatite/poly lactic acid (nHA/PLLA) composite and the compatibility study of it with osteoblast.Methods:nHA/PLLA composite was prepared by freezing rapid prototyping method;porosity was determined by BET;internal structure was observed by SEM to show physical properties;physical characteristics were presented through X-ray diffraction.The growth,adhesion and proliferation of osteoblasts in nHA/PLLA in vitro were shown by SEM;the amount of osteoblasts in nHA/PLLA were observed and calculated by inverted fluorescence microscope;the influence of nHA/PLLA composite on osteoblasts activity was determined by MTT.Results:nHA/PLLA composite was prepared with the pore size of (164±52) μm and the porosity of 89.3%±1.4%;the modulus of compression of dry and wet nHA/PLLA composite scaffolds were (1354.6±53.7) kPa and (1012.8±61.3) kPa,respectively;MTT results showed that nHA/PLLA composite had no significant influence on osteoblasts activity and with the prolongation of incubation,the amount of osteoblasts adhering to the composite surface and in the mesoporous increased gradually.Conclusions:The nHA/PLLA composite is a kind of scaffold material with high performance in bone tissue engineering,and its advantages include easy preparation,shape and internal structure meeting the requirements of tissue engineering scaffold and good biocompatibility with osteoblasts.