目的 制备环磷酰胺聚乳酸微球,并检验微球的体外缓释效果.方法 采用O/W型乳化溶剂挥发法制备载药微球,通过单因素实验优化制备工艺,用生物显微镜观察微球的形态,并用激光粒度分析仪分析粒径.用紫外-可见光谱法研究环磷酰胺聚乳酸微球的载药量、包封率和体外释药性能.结果 微球呈球形,直径为(12.67±3.67)μm.载药量和包封率分别为 15.38%和 62.5%,累计释放时间为 108h,累计释放率为84.07%.结论 本研究成功制备环磷酰胺聚乳酸微球,为制备可生物降解载药微球提供参考.
Objective To prepare cyclophosphamide polylactic acid microspheres and test the sustained release effect of microspheres in vitro.Methods Drug-loaded microspheres were prepared by O/W emulsion solvent evaporation method,and the preparation process was optimized by single factor experiment.The morphol-ogy of microspheres was observed by biomicroscope,and the particle size was analyzed by laser particle size an-alyzer.The drug loading,encapsulation efficiency and drug release in vitro of cyclophosphamide polylactic acid microspheres were studied by ultraviolet-visible spectrometry.Results The microspheres were spherical with a diameter of(12.67±3.67)μm.The drug loading and encapsulation rates were 15.38%and 62.5%,respec-tively,with a cumulative release time of 108 h and a cumulative release rate of 84.07%.Conclusion Cyclo-phosphamide polylactic acid microspheres were successfully prepared in this study,which provided a reference for the preparation of biodegradable drug-loaded microspheres.