目的:探讨老年多发性骨髓瘤患者的细胞遗传学改变与血清血管内皮生长因子(serum vascular endothelial growth factor,VEGF)以及血清酒石酸抗酸性磷酸酶(serum tartrate resistant acid phosphatase,TRacp-5b)水平的相关性。方法采用添加白细胞介素6的改良培养法对患者进行细胞遗传学分析,采用酶联免疫吸附法对患者血清进行 VEGF 及 TRacp-5b 的检测。结果在60例患者中发现细胞遗传学异常27例,其中22例涉及数目异常,15例涉及结构异常。有多例患者同时存在数目与结构异常。正常核型组33例,VEGF 水平为117.35±55.26 pg/mL,TRacp-5b 水平为4.15±2.15 U/L;异常核型组27例,VEGF 水平为190.26±85.74 pg/mL,TRacp-5b 水平为5.96±2.24 U/L,两项指标在两组之间的差异均有统计学意义(P <0.05)。结论在老年多发性骨髓瘤患者中,细胞遗传学异常组的血清VEGF 及 TRacp-5b 水平均远高于遗传学正常组。
Objective To assess the correlation of cytogenetic changes with serum vascular endothelial growth factor (VEGF)and serum tartrate resistant acid phosphatase (TRacp-5b)levels among elderly patients with multiple myeloma (MM).Methods Chromosomal changes were analyzed with a modified culturing method in the presence of IL-6.Serum levels of VEGF and TRacp-5b were determined with enzyme-linked immunosorbent assays (ELISA ). Results Among the 60 MM patients, chromosomal abnormalities were found in 27 cases,including 22 with numerical abnormalities and 1 5 with structural abnormalities.Many patients had both numerical and structural abnormalities.For 33 patients with a normal karyotype,the levels of VEGF and TRacp-5b were 1 1 7.35 ± 55.26 pg/mL and 4.1 5 ± 2.1 5 U/L, respectively,while for 27 patients with an abnormal karyotype,the levels of VEGF and TRacp-5b were 1 90.26 ± 85.74 pg/ml and 5.96 ± 2.24 U/L,respectively.The difference between the two groups was significant (P < 0.05 ).Conclusion Compared with MM patients with a normal karyotype,the levels of VEGF and TRacp-5b are higher in those with cytogenetic abnormalities.