目的:筛选中药Q0409改善小鼠学习记忆障碍的有效组方并进行验证.方法:腹腔注射东莨菪碱建立小鼠学习记忆障碍模型.各组小鼠每天同一时间给予相应药物灌胃,连续14 d.Morris水迷宫测定小鼠学习记忆能力,之后取其海马组织匀浆,测定其乙酰胆碱酯酶(AChE)活性.根据L8(27)正交表将动物分为8组,采用方差分析和析因分析的方法对中药Q0409组方的7种单味中药进行药效分析并确定初步筛选方;根据初筛结果,将动物分为6组,进一步检验和验证中药Q0409筛选方,并得出最终的精简方. 结果:通过Morris水迷宫和海马组织AChE活性正交实验结果的方差分析和析因分析,筛选出远志、人参和石菖蒲3味中药为筛选方;根据Morris水迷宫实验和海马组织AChE活性结果的方差分析剔除石菖蒲,得到中药Q0409的精简方.结论:最终确定中药Q0409精简方为人参和远志2味中药,该方具有较好的改善小鼠学习记忆障碍的效果.
AIM:To screen and verify the effective ingredient of traditional Chines medicine (TCM) Q0409 in improving learning and memory ability of mice.METHODS:The mouse learning and memory impairment model was induced by intraperitoneal injection of scopolamine.The mice in each group were given the corresponding drug by gavage at the same time for 14 d in succession.Morris water maze test was used to measure the learning and memory ability of the mice, and then the hippocampal tissue homogenate was taken to determine the activity of acetylcholinesterase (AChE).The animals were divided into 8 groups according to L8(27) orthogonal table.The variance analysis and factorial analysis were used to analyze the pharmacological effects of seven kinds of single herbal in TCM Q0409 and determine the screening ingredients.The animals were divided into 6 groups according to the results of the preliminary screening results, and further testing and validation of TCM Q0409 screening ingredients were performed to get the final simplified ingredients.RESULTS:Three medicinal herbs of Polygalae, Panax ginseng and Acori graminei rhizome were screened by the orthogonal results of Morris water maze test and the activity of AChE in mouse hippocampal tissues.The simplified ingredients of TCM Q0409 were obtained through the variance results of Morris water maze test and the activity of AChE in mouse hippocampal tissues.CONCLUSION:Polygala and ginseng were eventually determined as simplified ingredients of TCM Q0409 and it was verified that they improve the learning and memory ability of the mice with learning and memory impairment.