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요약정보
基于DNA条形码的甘草属植物的分子鉴别 / Molecular Identification in Genus of Glycyrrhiza Based on DNA Barcoding
Resource Type
Academic Journal
Authors
李珍珍
;
郑司浩
;
尚兴朴
;
邓庭伟
;
王继永
;
赵润怀
;
LI Zhen-zhen
;
ZHENG Si-hao
;
SHANG Xing-pu
;
DENG Ting-wei
;
WANG Ji-yong
;
ZHAO Run-huai
Source
中国现代中药 / Modern Chinese Medicine
. 22(2):207-218
Subject
甘草属
DNA条形码
分子鉴别
内转录间隔区
Language
Chinese
ISSN
1673-4890
Online Access
Full Text (CAJ - 경제/경영)
초록
目的:评价不同DNA条形码序列对甘草属的鉴定能力.方法:应用5条常用DNA条形码序列ITS、ITS2、psbA-trnH、matK与rbcL对甘草属进行PCR扩增与序列测序,结合在GenBank数据库下载的序列数据,通过分析各序列种内与种间变异与遗传距离,构建系统发育树,并基于相似性搜索算法和最近距离法计算各序列鉴定成功率,以分析比较各序列对甘草属的鉴定效率.结果:matk序列因扩增与测序成功率均较低,没有加入后续的数据分析.各个序列的鉴定成功率由高到低的顺序为psb A-trnH、ITS、ITS2、rbcL.ITS和ITS2序列种间最小变异均大于种内最大变异,且种内变异最小.Barcoding gap检验结果显示,ITS、ITS2的重叠区较小.NJ树结果显示,只有psbA-trnH序列能把甘草属不同物种分开.结论:利用DNA条形码序列可准确鉴别甘草属植物,psbA-trnH和ITS组合序列可作为鉴定甘草属植物的较优序列组合.本研究为甘草属植物的分子鉴别提供科学依据.
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