旨在研究泽泻粉对高能低蛋白日粮诱导的蛋鸡肝脏脂沉积的缓解作用.72羽260 d海兰褐蛋鸡随机分成3组,分别为对照组(CON,10.9 MJ/kg代谢能,16.9%粗蛋白),高能低蛋白日粮组(HF,13.0 MJ/kg代谢能,12.1%粗蛋白),以及高能低蛋白日粮添加泽泻粉组(HFA,13.0MJ/kg代谢能,12.1%粗蛋白,添加72 mg/kg泽泻粉).母鸡笼养,每日定量限饲(110 g/羽).鸡舍室温约24 ℃,光照16L:8D,试验期12周.结果表明:HF组产蛋率、蛋重、蛋品质指标,以及血浆谷丙转氨酶活性、总胆固醇和甘油三酯浓度与对照组相比均无显著差异(P>0.05),而肝脏中甘油三酯含量显著高于对照组(P<0.05);日粮添加泽泻粉显著缓解高能低蛋白日粮诱导的蛋鸡肝脏脂质沉积(P<0.05),同时显著逆转了肝脏脂质合成相关基因脂肪酸合酶、乙酰CoA羧化酶1和硬脂酰辅酶A去饱和酶-1 mRNA的上调,肝脏糖皮质激素受体(GR)mRNA和蛋白的上调也得到显著缓解(P<0.05).研究结果提示:泽泻通过抑制脂质从头合成基因的表达来缓解高能低蛋白日粮诱导的蛋鸡肝脏脂质沉积.在泽泻作用的信号通路中可能有GR的参与.
The aim of the trial was to explore the effect of Alisma powder in alleviating high energy and low protein diet-induced hepatic lipid deposition in laying hens.Seventy-two 260-day-old Hy-Line Variety Brown laying hens were obtained and randomly divided into three groups,namely,the control group(CON,treated with 10.9 MJ/kg metabolizable energy,16.9%crude protein),the high-energy and low-protein diet group(HF,with 13.0 MJ/kg metabolizable energy,12.1%crude protein),and the high-energy low-protein diet supplemented with Alisma powder group(HFA,with 13.0 MJ/kg metabolizable energy,12.1%crude protein,supplemented with 72 mg/kg Alisma pow-der).The hens were housed in cages and subjected to restricted feeding with 110 g/d/head.The room temperature of the chicken house was about 24 ℃,the light regime was 16L:8D,and the test period was 12 weeks.The results showed that the egg production rate,egg weight,egg quality index,plasma alanine aminotransferase activity,total cholesterol and triglyceride concentration in the HF group were not signifi-cantly different from those in the control group(P>0.05),whereas the content of triglycerides in their liver was significantly(P<0.05)higher than that of the control group;and adding Alisma powder to the diet significantly alleviated the hepatic lipid deposition of the laying hens,and at the same time significantly(P<0.05)reversed the up-regulation of fatty acid synthase,acetyl-CoA carboxylase 1 and stearoyl-CoA desaturase-1 mRNA of the hepatic lipid synthetic genes.Simultaneously,the upregulation of hepatic glucocorticoid receptor(GR)mRNA and protein was also significantly alleviated in the hens.These results suggested that Alisma alleviated hepatic lipid metabolic disorder induced by high-energy and low-protein diets by regulating de novo lipogenesis in laying hens.GR might be involved in the signaling pathway of the function of alisma.