目的 探讨血卟啉单甲醚声动力治疗(HMME-SDT)对增生性瘢痕成纤维细胞(Fb)的影响.方法 将兔增生性瘢痕Fb悬液按照对Fb悬液的处理方式分为四组:对照组、超声组、血卟啉单甲醚药物组、HMME-SDT组.采用MTT法检测兔增生性瘢痕Fb成活率.经流式细胞仪检测,罗丹明1 23检测线粒体膜电位,二氯荧光黄双乙酸盐检测细胞内活性氧,钙离子荧光探针检测细胞内钙离子浓度的变化,Hoechst33258核染色观察细胞核的形态改变.结果 MTT检测法显示HMME-SDT组细胞成活率与其他组间同一时间段比较,差异有统计学意义(P<0.01).经流式细胞仪检测,线粒体膜电位降低的细胞百分比、细胞内活性氧增高和细胞内钙离子增高的细胞百分比,HMME-SDT组与其他组相比,差异有统计学意义(P<0.01).结论 HMME-SDT能显著抑制增生性瘢痕Fb增殖活性.
Objective To investigate the mechanism of the effect of HMME-SDT on the proliferation of hypertrophic scar fibroblasts (Fb).Methods Fibroblast suspensions of rabbit ear with hypertrophic scar were divided into four groups.:control group,ultrasound group,HMME group and HMME-SDT group.The survival of the fibroblast suspension of rabbit ear were determined by MTT assay method.The mitochondrial membrane potential,cellular reactive oxygen,the concentration changes of cellular calcium ion were also investigated by flow cytometry correspondingly.Nucleolar morphology was observed by 33258 nucleoli stain.Results There was significant difference between the SDT group and control,HMME and ultrasound groups at same time (P< 0.01).There were significant differences between the HMME-SDT group and HMME,ultrasound and control groups on cellular reactive oxygen,nucelus concentration and floccuent change as well as nuclear margination.Conclusions HMME-SDT can inhibit the proliferation of hypertrophic scar fibroblasts of rabbit ear significantly.