目的 研究经后增殖方对超排卵大鼠卵巢颗粒细胞Fas凋亡通路的影响,探讨中药改善卵母细胞质量的作用机制.方法 建立超排卵大鼠模型,30只大鼠随机分为空白组、阳性组和中药组.实时荧光定量PCR和蛋白印迹法检测大鼠卵巢组织中Fas、Fasl、Caspase-8、Caspase-3基因及蛋白的表达.结果 阳性组各基因及蛋白表达均增高,与空白组比较差异具有显著性(P<0.05).中药组各基因及蛋白表达均低于阳性组,其中Fas、Fasl、Caspase-8表达水平与阳性组相比差异具有统计学意义(P<0.05),而Caspase-3表达水平与阳性组无统计学差异(P>0.05).中药组与空白组相比差异无统计学意义(P>0.05).结论 经后增殖方能抑制超排卵大鼠卵巢组织Fas、Fasl、Caspase-8、Caspase-3的表达,抑制颗粒细胞凋亡,推测Fas通路可能是经后增殖方改善卵母细胞质量的作用机制之一.
Objective To investigate the effect of Jinghou Zengzhi Recipe(JHZZR)on Fas apoptosis pathway in ovarian granulosa cells of rats with controlled ovarian hyperstimulation(COH)and to investigate the possible mechanism of the effect of JHZZR on improving oocyte quality. Methods To establish the model of COH,30 rats were randomly divided into the blank group,the positive group and the TCM group.The expression of Fas,Fasl,Caspase8,Caspase3 in ovarian was detected by qPCR andWestern Blot assay,respectively. Results The expression of Fas,Fasl,Caspase8,Caspase3 was lowered in the blank group and the TCM group. Conclusion JHZZR can inhibit Fas signalling,suggesting that Fas pathway may be one of the mechanisms under-lying that JHZZR improves oocyte quality.