目的:研究雪旺细胞(SCs)、神经干细胞(NSCs)与内置纵行排列纳米纤维丝的静电纺丝壳聚糖-聚羟基乙酸(Chitosan-PLGA)纳米导管材料的细胞相容性,探讨细胞在导管材料上的黏附及定向生长情况。方法实验分为 SCs 组、NSCs 组、SCs-NSCs 共培养组,3组细胞分别与 Chitosan-PLGA 纳米导管材料复合培养,四甲基偶氮唑蓝(MTT)比色法观察各组细胞存活率,扫描电镜下观察 SCs、NSCs 在 Chitosan-PLGA 上的生长。结果细胞存活率排序由高至低依次为共培养细胞组、SCs 组、NSCs 组。SCs 及 NSCs 能贴附在 Chitosan-PLGA 内置纳米纤维丝上生长并沿纤维丝定向生长。结论雪旺细胞及神经干细胞共培养可促进细胞与 Chitosan-PLGA 材料的相容性。内置纵行排列的纳米纤维丝有引导细胞定向生长与迁移的作用。
Objective To investigate cytocompatibility of the electron spinning chitosan-PLGA nerve conduits with Schwann cells (SCs)and neural stem cells(NSCs)as well as cells adhesion and migration on the arranged nanofibers inside the lumen.Methods Experiment was divided into three groups:the SCs group,the NSCs group and the SCs-NSCs co-culture group.Cells in these groups were co-seeded with Chitosan-PLGA.The biocompatibility and absorba-bility of SCs and NSCs were detected by MTT assays.Scanning electron microscopy(SEM)was used to detect cells ad-hesion and migration on the axially arranged nanofibers inside the lumen.Results Cell survival rate of the co-culture group was the highest,the higher in the SCs group,and the lowest in the NSCs group.SCs and NSCs had good compati-bility and adhesion with Chitosan-PLGA,and the axial nanofibers in the lumen guided the growth of neurites in a particu-lar direction.Conclusion Co-culture of SCs and NSCs can facilitate cells compatibility and adhesion with 3D Chitosan-PLGA,and the axial arranged nanofibers within the lumen has the effect of directional guide cell growth and migration.