目的:评价改进后的小脑延髓池穿刺法制作神经源性肺水肿大鼠模型的使用价值。方法60只清洁级SD大鼠随机均分为4组:正常对照组、假手术组、小脑延髓池穿刺直接注射组(直接注射组)、小脑延髓池穿刺延长留置时间组(延长留置时间组)。延长留置时间组造模成功率达90%。直接注射组采用传统的小脑延髓池穿刺直接注射法造模;延长留置时间组即小脑延髓池穿刺成功后,在脑池内留置1 mL注射器针头20 min后注射纤维蛋白原(100 mg/mL)和凝血酶(200 U/mL)各0.075 mL。通过观察各组大鼠肺大体形态学、病理改变及比较生存时间,评价两种制模方法的价值。正常对照组不予任何干预。结果正常对照组无肺水肿表现。同直接注射组相比,延长留置时间组大鼠肺体积明显增大,肺泡及肺间质渗出明显增多。延长留置时间组大鼠生存时间较直接注射组缩短,分别为5.008±2.612 h和7.482±3.034 h(t=2.394,P=0.024)。结论小脑延髓池穿刺延长留置时间法复制的神经源性肺水肿症状典型,是研究神经源性肺水肿较为理想的动物模型。
Objective To evaluate the worth of neurogenic pulmonary edema model induced by improved cerebel-lomedullary cistern puncture. Methods 60 SD ras were randomly divided into four groups:control group(n=15);Sham operation group( n=15 );direct injection through cerebellomedullary cistern puncture group ( abbreviated:direct injection group,n=15);prolonging the indwelling time and injection through cerebellomedullary cistern puncture group(abbreviated:prolonging indwelling time group,n=15). 90% of them is successful in the prolonging indwelling time group. Neurogenic pulmonary edema model was induced by classic cerebellomedullary cistern puncture in the direct injection group,however, that in the prolonging indwelling time group was induced by injecting 0. 75 mL Fibrinogen(100 mg/mL) and 0. 75 mL thrombin (200 U/mL) respectively after 20 min indwelling needle maintained in cerebellomedullary cistern. The experimen-tal parameters including pulmonary morphology,pathology change and survival time in each group were evaluated. Results The pulmonary edema of rats did not be discovered in the control group,however,that in the direct injection group and pro-longing indwelling time group were both discovered significantly. Compared with the rats in the direct injection group,the lung volume,exudation of the pulmonary alveoli and pulmonary interstitium increased significantly in the prolonging indwell-ing time group. Survival time of the rats in the prolonging indwelling time group was shorter than that of the direct injection group,they are (5. 008+2. 612) h and (7. 482+3. 034) h,respectively,(t=2. 394,P=0. 024). Conclusion It is perfect NPE model for research that the modified NPE model has typical NPE symptoms.