为明确海南基地茄子根结线虫病原种类并筛选抗病砧用茄子种质,采用形态学和rDNA-ITS分子生物学鉴定病原线虫种类,通过病土苗期鉴定与田间病圃诱发鉴定2种方法对58份砧用茄子种质进行抗性筛选.结果表明,具有方形背弓的南方根结线虫检出频率为93.33%,而花生根结线虫检出频率为6.67%;NCBI数据库比对分析显示,用南方根结线虫特异性引物扩增的序列与南方根结线虫KP411871.1的一致性为99.89%,用花生根结线虫特异性引物扩增的序列与花生根结线虫MW315990.1的一致性为99.76%;因此,确定南方根结线虫是侵染海南茄子的优势类群.2种方法鉴定参试茄子种质的根结线虫整体病情程度基本一致,苗期鉴定的总病情指数高于田间鉴定,筛选出高抗根结线虫砧用茄子种质6份,分别为水茄、蒜芥茄、LJ-7、Ng1-039、Ng1-021、Ng1-003.
To identify the species of root-knot nematodes(Meloidogyne spp.)of eggplant in Hainan base and select resistant germplasms of eggplant for rootstock.The morphological characteristics and rDNA-ITS molecular biological identifi cation of root-knot nematodes on eggplant were both adopted in this study.Two methods(seedling inoculation and fi eld trials)were used to screen germplasms with high resistance to root-knot nematodes from 58 materials of eggplant for rootstock.The isolation frequency of M.incognita with square dorsal arch was 93.33%,while M.arenaria was 6.67%.NCBI sequences blast showed 99.89%similarity with M.incognita(KP411871.1)by M.incognita primer,and 99.76%similarity with M.arenaria(MW315990.1)by M.arenaria primer.Therefore,M.incognita was the predominant species infecting eggplant in Hainan base.The overall disease severity of the tested germplasms were basically consistent between the two methods.The total disease index of seedling identifi cation was higher than that of fi eld trials.Six eggplant for rootstock germplasms with high resistance to root-knot nematode were screened,which were S.torvum Swartz,S.sisymbriifolium Lam,LJ-7,Ng1-039,Ng1-021,Ng1-003,respectively.