目的 建立向天果高效液相色谱(HPLC)指纹图谱及2种柠檬苦素类成分含量测定方法.方法 采用KR100-5 C18(250 mmx4.6 mm)色谱柱,以乙腈(A)-水(B)为流动相进行梯度洗脱,柱温30 ℃,流速1 ml/min,检测波长210 nm.采用《中药色谱指纹图谱相似度评价系统(2012版)软件》,结合聚类分析和主成分分析比较30批向天果色谱图,同时测定了向天果中swietenolide和swietwnine的含量.结果 建立了向天果HPLC指纹图谱,并对13个共有峰中的5个主要共有峰进行指认.30批向天果swietenolide和swietwnine的平均含量分别为14.28,10.74 mg/g.结论 本文所建立的指纹图谱和含量测定方法可用于向天果的质量控制.
Objective To establish the HPLC fingerprints of Fructus Swietenia macrophylla and determine the content of 2 kinds of limonoids.Methods The KR100-5 C18(250 mmx4.6 mm)column was adopted,a gradient elution was performed,using acetonitrile(A)-water(B)as mobile phase.The detection wavelength was set at 210 nm,the column temperature was 30 ℃,and the flow rate was 1 ml/min.The chromatograms of 30 batches of Fructus Swietenia macrophylla were compared by the software of Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine(2012 Version),combined with cluster analysis and principal component analysis.The contents of swietenolide and swietwnine were also determined simultaneously.Results The HPLC fingerprint of Fructus Swietenia macrophylla with 13 common chromatographic peaks was constructed,five chromatographic peaks of which were identified as limonoids.The average contents of swietenolide and swietwnine in 30 batches of Fructus Swietenia macrophylla were 14.28 mg/g and 10.74 mg/g,respectively.Conclusion Established HPLC fingerprint and content determination method of 2 kinds of limonoids can be used for quality control of Fructus Swietenia macrophylla.