以多聚核苷酸Oligo-pTa535-1和Oligo-pSc119.2-1为探针对济麦系列小麦进行双色荧光原位杂交( FISH)分析,结果发现, Oligo-pTa535-1的FISH信号主要分布在 A 和 D 染色体上,而Oligo -pSc119.2-1的FISH信号主要分布在B染色体上;济麦系列小麦的FISH位点较小麦中国春差异位点主要集中在染色体4A、6B、7B和1D染色体上,且多为Oligo-pSc119.2-1信号;济南17的1B染色体着丝粒区Oligo-pTa535-1信号、济麦20的1D短臂末端和2A长臂末端的Oligo-pSc119.2-1信号、济麦262的5A长臂中间的Oligo-pSc119.2-1信号可分别作为细胞学标签用于其身份识别。济麦系列小麦标准FISH核型的建立为利用染色体工程对其进行改良奠定了良好的细胞遗传学基础;FISH信号变异位点的类似性,说明济麦系列小麦的遗传基础相对狭窄,应在今后育种工作中注意选择与其亲缘关系相对较远的育种亲本进行组配。
In this research, dual-color fluorescence in situ hybridization ( FISH) on Jimai serial wheat was performed using Oligo-pTa535-1 and Oligo-pSc119.2-1 as probes.As a result, the FISH signals of Oligo-pTa535-1 mainly distributed on chromosome A and D, while the FISH signals of Oligo-pSc119 .2-1 mainly distributed on chromosome B.The standard FISH patterns of Jimai varieties ( lines) were somehow different from those of Chinese Spring, and the different FISH sites were mainly distributed on chromosome 4A, 6B, 7B and 1D, mostly were signals of Oligo-pSc119.2-1.The signal of Oligo-pTa535-1 on cen-tromeric region of chromosome 1B of Jinan17, and the signals of Oligo-pSc119.2-1 on terminal region of chromosome 1D short arm and chromosome 2A long arm of Jimai20 could be regarded as their cytology tags. The establish of standard FISH patterns of Jimai serial wheat laid good cytogenetic foundation for wheat im-provement through chromosome engineering.The similarity of FISH signal variation positions of Jimai serial wheat indicated a narrow genetic bases of them.Therefore, Jimai serial wheat should cross with parents pos-sessing relatively distant genetic relationship in wheat breeding in the future.