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요약정보
猪瘟、猪蓝耳病和乙型脑炎多重RT-PCR检测方法的建立及临床验证
Resource Type
Academic Journal
Authors
王迎平
;
李有豪
;
曹德琴
;
黄茹芸
;
李照伟
;
浦同灿
;
王慧
;
曾红
;
杨晓伟
;
刘霞
;
赵光伟
Source
贵州畜牧兽医 / GUIZHOU JOURNAL OF ANIMAL HUSBANDRY & VETERINARY MEDICINE
. 47(1):52-56
Subject
猪瘟
猪蓝耳病
乙型脑炎
多重RT-PCR
Language
Chinese
ISSN
1007-1474
Online Access
Find it @ DONGA
초록
为了提高猪繁殖障碍性疾病的检测效率,试验在单一RT-PCR扩增条件的基础上,建立1种能同时检测猪瘟病毒(CSFV)、猪蓝耳病病毒(PRRSV)、乙型脑炎病毒(JEV)的多重RT-PCR方法,并在四川省的简阳、宜宾、泸州地区11个养殖场进行临床验证.结果:多重RT-PCR方法对CSFV、PRRSV、JEV的最低检测限分别为0.1696、1.209、1.678 ng/μL,检测结果与商品化试剂盒检测结果均一致.结论:建立的多重RT-PCR方法能够同时检测3种繁殖障碍病毒,灵敏度高、特异性好,可应用于实验室检测诊断.
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