目的:探讨微小 RNA-22(miRNA-22)在胃癌组织中的表达及与胃癌临床病理特征间的关系。方法采用实时定量 PCR(RT-PCR)法检测88例胃癌组织与相应癌旁组织中 miRNA-22的表达水平,分析miRNA-22表达水平与胃癌患者临床病理特征之间的关系。结果87.5%(77/88)的胃癌组织中 miRNA-22相对表达量明显低于相应正常癌旁组织,差异有统计学意义(χ2=69.32,P <0.05)。T3、T4期患者中 miRNA-22低表达率为66.7%(34/51),显著高于 T1、T2期患者的27.0%(10/37),差异有统计学意义(χ2=64.38,P <0.05);有淋巴结转移者低表达率为72.9%(35/48),显著高于无淋巴结转移者的27.5%(11/40),差异有统计学意义(χ2=68.56,P <0.05);miRNA-22表达率与性别、年龄、分化程度无相关性(均 P >0.05)。结论miRNA-22在胃癌组织中的表达下调与胃癌的临床病理特征密切相关。
Objective To investigate the expression and clinical value of miRNA -22 in gastric cancer tissues.Methods Real -time PCR was used to detect the miRNA -22 expression in gastric cancer tissues and matched adjacent tissues.The correlations of miRNA -22 expression with clinic pathological features were analyzed. Results 87.5%(77 /88)of the gastric tumor tissues showed aberrant down regulation of miRNA -22 compared with adjacent non -tumor tissues (χ2 =69.32,P <0.05).Lower miRNA -22 level was strongly associated with T3 -T4 stage (χ2 =64.38,P <0.05)and lymph node metastasis(χ2 =68.56,P <0.05).The correlation statistical analysis results indicated that there were no significant differencec in sex,age and differentiation grade (all P >0.05 ). Conclusion The lower expression of miRNA -22 is associated with clinic pathological features.