为评价耐盐碱微生物对盐胁迫下植物的促生效应,本文以1年生"阳光玫瑰"葡萄为试验材料,利用盆栽试验评估2株耐盐碱芽胞杆菌菌株(HMB38720和HMB38667)对葡萄生长及生理特性的影响.研究结果表明,与对照相比,在盐胁迫末期(44 d,6次灌施后10 d),灌根使用2株耐盐碱芽孢杆菌菌株后,葡萄新梢相对生长幅度显著提高了 77.37%和198.69%,叶面积显著提高了 50.61%和38.35%,葡萄叶片SPAD值显著提高了 22.40%和16.60%,而叶片相对电导率显著降低了 30.00%和23.50%,施用菌株HMB38720后叶片丙二醛(MDA)含量显著降低18.10%;菌株HMB38720处理对育苗基质电导率和pH值无影响,而菌株HMB38667处理后这2种指标显著下降了 19.17%和0.32.同时芽孢杆菌菌株均能明显或显著增加葡萄叶片中防御酶(SOD,POD,PAL,PPO)的活性.冗余分析表明,植物生长表型(叶片面积和叶片SPAD值、新梢相对生长幅度)与叶片的4种防御酶活性呈正相关.研究结果表明,2株耐盐碱芽孢杆菌菌株均能够缓解葡萄植株遭受盐胁迫的损害,具有生产应用潜力.
In order to evaluate the growth-promoting effects of salinity-tolerant microorganisms on plants under salt stress,the effects of two strains of Bacillus subtilis(HMB38720 and HMB38667)on the growth and physiological characteristics of grapes under salt stress were e-valuated by pot experiment with 1-year-old Sunshine Rose grapes as the test material.The re-sults showed that compared with the control,at the end of salt stress treatment(44 d,10 d af-ter 6 times of irrigation),after the use of 2 salinity-tolerant Bacillus strains in the irrigations,the relative growth rate of new shoots,the leaf area,the SPAD value of grape leaves was signif-icantly increased by 77.37%and 198.69%,50.61%and 38.35%;22.40%and 16.31%,respec-tively,while leaf relative conductivity was significantly reduced by 30.00%and 23.50%,respec-tively,and leaf malondialdehyde(MDA)content was significantly reduced by 18.10%after the application of strain HMB38720.The treatment of strain HMB38720 had no effect on the seed-ling substrate conductivity and pH,while these two indexes were significantly reduced by 19.17%and 0.32,respectively after the treatment with strain HMB38667.Meanwhile,all Bacil-lus strains obviously or significantly increased the activities of defense enzymes(SOD,POD,PAL,PPO)in grape leaves.Redundancy analysis showed that plant growth phenotypes(leaf area and leaf SPAD values,relative growth rate of new shoots)were positively correlated with the activities of four defense enzymes in leaves.The results of the study indicated that both sa-linity-tolerant Bacillus strains were able to alleviate the damage of grapevine plants subjected to salt stress,and have potential for application in grape production.