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요약정보
CXCL2在巨核细胞分化过程中的功能研究 / Research on the Function of CXCL2 During Megakaryocyte Differentiation
Resource Type
Academic Journal
Authors
王迪
;
赵艳红
;
梁一鹏
;
宋昊泽
;
石莉红
;
佟静媛
;
WANG Di
;
ZHAO Yanhong
;
LIANG Yipeng
;
SONG Haoze
;
SHI Lihong
;
TONG Jingyuan
Source
生物技术进展 / CURRENT BIOTECHNOLOGY
. 13(2):257-263
Subject
巨核细胞分化
CXCL2
CD34+细胞
Language
Chinese
ISSN
2095-2341
Online Access
Find it @ DONGA
초록
临床中血小板输注供需矛盾愈发尖锐,体外血小板生成的相关研究在全球受到广泛关注,主要集中于干细胞来源、细胞因子、转录因子、湍流体系等方面.由于血小板由成熟巨核细胞释放产生,提高巨核细胞分化效率,将有利于体外生产血小板体系的优化.CXCL2具有促炎、促进血管生成的作用,与心血管疾病、结肠癌进展等有关,但其在巨核细胞分化过程中的功能尚不清楚.利用脐带血CD34+细胞向巨核细胞诱导分化体系,通过慢病毒介导的基因敲降方法降低CXCL2的表达水平,并利用流式细胞术检测巨核细胞的分化效率.结果表明,CXCL2被敲降后,CD34+细胞向巨核细胞的早期增殖分化受到抑制,并且这一抑制效应在增殖分化过程中持续存在.综上,CXCL2在巨核分化过程中发挥着重要的调控作用,这一结论将对体外巨核分化及生产血小板具有一定的指导意义.
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