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요약정보
AlphaLISA检测新型冠状病毒 / AlphaLISA for detection of SARS-CoV-2
Resource Type
Academic Journal
Authors
曹鹤译
;
邓永强
;
刘鹏
;
江华
;
孔德聪
;
律清宇
;
姜永强
;
CAO He-yi
;
DENG Yong-qiang
;
LIU Peng
;
JIANG Hua
;
KONG De-cong
;
Lü Qing-yu
;
JIANG Yong-qiang
Source
军事医学 / Military Medical Sciences
. 45(11):848-851
Subject
均相光激化学发光免疫分析法;新型冠状病毒;酶联免疫吸附测定
Language
Chinese
ISSN
1674-9960
Online Access
Find it @ DONGA
초록
目的 建立均相光激化学发光免疫分析方法(AlphaLISA)快速检测新型冠状病毒(SARS-CoV-2).方法 采用新型冠状病毒抗体偶联发光微球,生物素标记另一抗体,以及链霉亲和素偶联感光微球构建新型冠状病毒AlphaLISA检测体系.采用SARS-CoV-2真核表达抗原和灭活病毒对AlphaLISA方法进行评价,并与商业化ELISA试剂盒进行比对.结果 确定单抗1#与多抗组别作为新型冠状病毒AlphaLISA检测的抗体对,AlphaLISA对新型冠状病毒抗原的检出限为0.39 ng/ml,具有较好的重复性,批内差1.90%~8.93%,批间差1.75%~9.04%,对不同临床样本的检测具有较好的耐受性,与ELISA试剂盒相比对灭活病毒的检测敏感性更高.结论 AlphaLISA可实现对SARS-CoV-2的高效快速检测.
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