条锈病是四川小麦重要病害.为了解四川近年来审定小麦品种的条锈病抗性水平和抗病基因利用情况,对2017年以来审定的63个小麦新品种进行条锈病抗性鉴定,利用抗病基因Yr5、Yr7、Yr15、Yr18、Yr27、Yr28、Yr36、Yr46、YrU1、YrSP的功能标记和Yr17、Yr26、Yr29的紧密连锁标记进行检测.结果表明,57个品种在苗期对条锈菌小种CYR34的抗性水平达到中抗以上(IT=0~6).所有品种的田间成株期抗性均达到中抗及以上(IT=1~4).未检测到Yr5、Yr27、Yr46和YrU1的功能位点,其余9个基因均存在于四川小麦品种中.Yr7、Yr17、Yr26和Yr29在供试品种的分布频率超过50%.综上所述,四川小麦新品种条锈病抗性水平整体较高,条锈病抗性基因丰富;小麦祖先物种抗条锈病基因Yr15、Yr28和Yr36已成功应用于四川小麦育种.
Wheat stripe rust is an important disease in Sichuan Province.In order to analyze the stripe rust resistance and the distribution of disease resistance genes in wheat cultivars of Sichuan Province,stripe rust resistance tests were performed at both seedling and adult plant stages with 63 Sichuan wheat cultivars released since 2017 as materials.The gene-based markers of Yr5,Yr7,Yr15,Yr18,Yr27,Yr28,Yr36,Yr46,YrU1 and YrSP as well as linkage molecular markers of Yr17,Yr26 and Yr29 were used to detect resistance genes in these cultivars.A total of 57 out of the 63 wheat cultivars showed intermediate to high stripe rust resistance(infection type,IT=0-6)against CYR34 at see-ding stage.All of them had moderate to high stripe rust resistance(IT=1-4)at adult plant stage in the field.Nine Yr genes were detected in different wheat cultivars,while Yr5,Yr27,Yr46 and YrU1 were absent in the tested cultivars.The distribution frequencies of Yr7,Yr17,Yr26 and Yr29 in Si-chuan wheat cultivars were more than 50%.Taken together,it was shown that the Sichuan wheat cultivars carry diverse stripe rust resistance genes that provide adequate resistance to stripe rust.The stripe rust resistance genes Yr15,Yr28,and Yr36 from wheat ancestor species have been successfully used in Sichuan wheat breeding.