目的 研究不育男性精液质量与细菌感染之间的相关性,为指导不育男性人群的生殖健康提供临床依据.方法 采集不育男性精液200 例作为研究对象,然后进行分离培养48 h,根据培养结果,分为无致病菌生长组、有致病菌生长组、无菌生长组,完成有致病菌生长组细菌耐药性分析,并进行各组间精液质量比较.结果 分离培养48h后,200 例精液中无致病菌生长 163 例,占比81.5%,有致病菌生长33 例,占比 16.5%,无菌生长4 例,占比2.0%;33 例有致病菌培养结果前三位依次为大肠埃希菌、无乳链球菌和粪肠球菌,耐药率分别为 80.0%、87.5%、100.0%.结论 不育男性精液中细菌培养检出率较高,且致病细菌会对精液质量产生影响.
Objective To study the correlation between semen quality and bacterial infection in men with abnormal fer-tility,and provide clinical basis for guiding the reproductive health of men with abnormal fertility.Methods 200 male semen samples with abnormal fertility were collected,and then separated and cultured for 48 hours.According to the culture results,they were divided into three groups:the non-pathogenic group,the pathogenic group,and the sterile group.The bacterial resist-ance analysis of the pathogenic group was conducted,and the semen quality between each group was compared.Results After 48 hours of isolation and cultivation,200 semen samples had been tested,non-pathogenic bacteria was detected in 163 semen samples,accounting for 81.5% ;pathogenic bacteria was detected in 33 semen samples,accounting for 16.5% ;and bacteria was not detected in 4 semen samples,accounting for 2.0% .The top three strains of pathogenic bacteria in 33 cases were Escherichia coli,Streptococcus agalactiae,and Enterococcus faecalis,with drug resistance rates of 80.0% ,87.5% ,and 100.0% ,respec-tively.Conclusion The detection rate of bacterial culture in semen of men with abnormal fertility is relatively high,and patho-genic bacteria can affect semen quality.