目的 基于UHPLC-Q-Orbitrap/MS分析不同产地(吉林、辽宁、黑龙江、山东)西洋参皂苷类成分.方法 运用UHPLC-Q-Orbitrap/MS法定性分析西洋参中皂苷类成分,结合主成分分析、正交偏最小二乘法判别分析、差异成分分析筛选各产地的差异性皂苷.结果 共得到 62 个皂苷类化合物.主成分分析表明不同产地西洋参中所含皂苷类成分存在差异,正交偏最小二乘判别分析进一步筛选出 28 个差异皂苷类化合物,包括原人参二醇型 13 个、原人参三醇型 6 个、齐墩果烷型 4 个、奥克梯隆型 2 个、C-17 侧链变异型 3 个;原人参二醇型和原人参三醇型皂苷类成分在 4 个产地相对含量由高到低依次为辽宁、吉林、黑龙江、山东.结论 该方法简便、准确、高效,可为西洋参的质量评价提供理论依据.
AIM To analyze saponins from Panacis Quinquefolii Radix of different regions(Jilin,Liaoning,Heilongjiang,Shandong)based on UHPLC-Q-Orbitrap/MS technology.METHODS Panacis Quinquefolii Radix had its saponins qualitatively analyzed by UHPLC-Q-Orbitrap/MS;and its differential saponins revealing different regions screened by the principal component analysis,orthogonal partial least squares discriminant analysis and differential component analysis.RESULTS A total of 62 saponins were identified;saponin variations due to the growth areas verified by the principal component analysis;and 28 differential saponins including 13 protopanaxadiol,6 protopanaxatriol,4 oleanolane,2 oxytetracycline,and 3 C-17 side chain variants further screened by orthogonal partial least-squares discriminant analysis model.The relative contents of protopanaxadiol-type and protopanaxatriol-type saponins in the four producing areas reduced following the order of Liaoning,Jilin,Heilongjiang,and Shandong.CONCLUSION This simple,accurate and efficient method provides a theoretical basis for the quality evaluation of Panacis Quinquefolii Radix.