试验以竹燕窝(海绵胶煤炱菌)为材料开发新型饲料添加剂.采用正交试验确定竹燕窝提取物的最佳提取工艺条件.采用牛津杯法探讨竹燕窝乙醇提取物对3种革兰氏阳性菌[粪肠球菌(Enterococcus faecalis)、金黄色葡萄球菌(Staphylococcus aureus)、表皮葡萄球菌(Staphylococcus epidermidis)]和3种革兰氏阴性菌[鼠伤寒沙门氏菌(Salmonella typhimurium)、鸡沙门氏菌(Salmonella pullorum)、大肠杆菌(Escherichia coli)]的抑菌效果.结果表明,竹燕窝抑菌提取物的最优提取工艺为乙醇浓度85%、液料比5 mL/g、提取时间180 min、提取温度50℃.竹燕窝乙醇提取物对革兰氏阳性菌的抑菌效果优于革兰氏阴性菌.研究表明,试验明确了竹燕窝在抑菌方面的潜在应用价值,可为抑菌产品的开发提供参考.
The experiment used Scorias spongiosa as the material,to develop a new limiting resistance feed additive.An orthogonal test was used to determine the optimum extraction conditions of Scorias spongiosa extract.The Oxford cup method was employed to investigate the antibacterial effects of ethanol extracts from Scorias spongiosa on three Gram-positive bacteria(Enterococcus faecalis,Staphylococcus aureus,and Staphylococcus epidermidis)and three Gram-negative bacteria(Salmonella typhimurium,Salmonella pullorum,and Escherichia coli).The results indicated that the optimal extraction process for Scorias spongiosa inhibitory extracts was an ethanol concentration of 85%,a liquid-to-material ratio of 5 mL/g,an extraction time of 180 minutes,and an extraction temperature of 50℃.The inhibitory effect of Scorias spongiosa ethanol extracts on Gram-positive bacteria was superior to that on Gram-negative bacteria.The study clarifies the potential application value of Scorias spongiosa in terms of antibacterial properties,providing reference for the development of antibacterial products.