目的:比较30K液相芯片和50K液相芯片的性能.方法:该研究收集共322头纯种大白猪和杜洛克猪的生长性能测定记录,使用30K液相芯片和50K液相芯片对其中177头进行基因组分型,用358头大白猪、长白猪和杜洛克猪的重测序数据作为填充参考群体进行填充,分析2款芯片的填充效果,利用2款芯片的原始和填充数据对日增重、日采食量和饲料转化率进行全基因组关联分析,并对比3个性状的GWAS结果.结果:30K液相芯片的参考填充准确率高于50K液相芯片,50K液相芯片的自填充GWAS分析结果优于30K液相芯片,而参考填充GWAS分析效果,50K液相芯片发现位点较多,但整体情况30K液相芯片较好.结论:30K液相芯片在分析过程中具有整体上的优势.
Objective:This study aimed to compare the performance of the 30K liquid chip and the 50K liquid chip.Method:A total of 322 purebred Duroc and Landrace pigs were collected and their growth performance records were obtained.Among them,177 pigs were genotyped using the 30K liquid chip and the 50K liquid chip.The sequencing data of 358 Duroc,Large White,and Landrace pigs were used as reference populations for imputation.The imputation effects of the two chips were analyzed,and the whole-genome association analysis was performed on daily weight gain,daily feed intake,and feed conversion ratio using the original and imputed data from the two chips.The GWAS results of the three traits were compared.Results:The reference-imputation accuracy of the 30K liquid chip was higher than that of the 50K liquid chip.The self-imputation GWAS analysis results of the 50K liquid chip were better than those of the 30K liquid chip.However,in terms of reference-imputation GWAS analysis,the 50K liquid chip discovered more markers,but overall,the 30K liquid chip performed better.Conclusion:The 30K liquid chip has overall advantages in the analysis process.