Proteomic analysis of human thyroid fine needle aspiration fluid
- Resource Type
- Authors
- Federica Ciregia; Paolo Miccoli; Fulvio Basolo; Gino Giannaccini; Pietro Iacconi; Antonio Lucacchini; Gianluca Donatini; Laura Giusti
- Source
- Scopus-Elsevier
- Subject
- Adult
Male
Proteomics
Pathology
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Biopsy, Fine-Needle
Thyroid Gland
Biology
Peptide Mapping
Endocrinology
Peptide mass fingerprinting
medicine
Humans
Electrophoresis, Gel, Two-Dimensional
Protein pattern
skin and connective tissue diseases
neoplasms
Aged
Gel electrophoresis
medicine.diagnostic_test
Thyroid
Middle Aged
Thyroid Diseases
Functional system
Body Fluids
body regions
surgical procedures, operative
Fine-needle aspiration
medicine.anatomical_structure
Proteome
Female
Human thyroid
Biomarkers
- Language
The aim of this study was to determine the protein pattern of human thyroid fine needle aspiration fluid (FNA) using a proteomic approach. FNA proteins were separated using 2-dimensional gel electrophoresis (2DE), digested and then analyzed by peptide mass fingerprinting. For the first time, we provided an image of the protein components of the FNA, in which approximately 220 protein spots can be identified. The proteome analysis revealed a specific fingerprint of FNA with proteins appertaining to various functional systems. Our preliminary results of FNA protein pattern could be a starting point in studying the presence of potential markers implicated in thyroid diseases.