Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation
- Resource Type
- Authors
- Masaaki Tokuda; Ryoji Konishi; Yuji Tai; Ryoji Kobayashi; Toyohiro Tsumura; Fuminori Yamaguchi; Takaya Gotoh; Katsuyoshi Sugimoto; Bushra Y. Ahmed
- Source
- Neuroscience letters. 290(2)
- Subject
- Neurons
endocrine system
Calmodulin
biology
Kinase
musculoskeletal, neural, and ocular physiology
General Neuroscience
Long-Term Potentiation
Long-term potentiation
Hippocampal formation
In Vitro Techniques
Subcellular localization
Hippocampus
Cell biology
Rats
Rats, Sprague-Dawley
Calmodulin dependent protein kinase
nervous system
Cytoplasm
Calcium-Calmodulin-Dependent Protein Kinases
biology.protein
Animals
Female
Protein kinase A
Neuroscience
- Language
- ISSN
- 0304-3940
Long-term potentiation (LTP) is considered to be associated with an increase in expression as well as activity of Ca 2+ /calmodulin-dependent protein kinases (CaMKs). LTP-induced and control hippocampal slices were studied by immunohistochemical and electronmicroscopic analyses using anti-CaMK-I, -II and -IV antibodies. All three kinases were demonstrated to increase their expression in CA1 neurons. CaMK-I was shown to mainly localize in the cytoplasm of the control and LTP-induced neurons, and a significant increase of immunoreactivity was observed in the latter neurons. A part of CaMK-I was found to translocate to the nuclei of LTP-induced hippocampal CA1 neurons. Direct evidence of the translocation of CaMK-II from cytoplasm to nuclei in LTP was demonstrated by immuno-electronmicroscopy. A significant increase in expression of CaMK-IV in the nuclei was also observed. Our data suggest that all the three CaMKs were actively involved in nuclear Ca 2+ -signaling in LTP.