Short Communication: Novel Single Nucleotide Polymorphisms (SNPs) in the 5'UTR of Bovine Heat Shock Protein 70 (bHSP70) Gene and its association with Service per Conception (S/C) of Pasundan cattle
- Resource Type
- Authors
- Widya Pintaka Bayu Putra; Syahruddin Said
- Source
- Biodiversitas, Vol 19, Iss 5, Pp 1622-1625 (2018)
- Subject
- 0301 basic medicine
Candidate gene
pic
QH301-705.5
Single-nucleotide polymorphism
Plant Science
Biology
03 medical and health sciences
Genotype
Insertion
Biology (General)
Molecular Biology
Gene
Genetics
Haplotype
0402 animal and dairy science
Heterozygote advantage
snp
04 agricultural and veterinary sciences
040201 dairy & animal science
Hsp70
030104 developmental biology
pasundan cattle
Animal Science and Zoology
s/c
bhsp70 gene
- Language
- English
- ISSN
- 2085-4722
Said S, Putra WPB. 2018. Short Communication: Novel Single Nucleotide Polymorphisms (SNPs) in the 5'UTR of Bovine Heat Shock Protein 70 (bHSP70) Gene and its association with Service per Conception (S/C) of Pasundan cattle. Biodiversitas 19: 1622-1625. Heat stress in the livestock reduces reproductive traits, including of service per conception (S/C). The influence of heat stress in livestock can be handled through molecular selection. One of the candidate genes that is affecting heat stress tolerance is Bovine Heat Shock Protein 70 (bHSP70) gene. This research was carried out to detect the single nucleotide polymorphism (SNPs) in the 5'UTR of bHSP70 gene from 44 heads of Pasundan cows at breeding station (BPPIBT-SP Ciamis, West Java). Research showed that 18 SNPs were detected with sequencing method. The insertion mutation was detected in all animal studied and occurred at position between g.1112 and g.1113. The moderate of polymorphic informative content (PIC) were detected in two SNPs of g.1117G/A and g.1125A/C. Preliminary analysis showed that haplotype of two homozygote genotype combination from both SNPs had better of S/C value of than the others (P