PCR amplification of Rad51C-ATXN7 fusion DNA: Genomic DNA was isolated from the LS 174 T, MCF-7, RKO, tumors (T) and non-tumors (NT). PCR was and performed using forward primer (sequence 5′-GCCCTGGGTTTTAAGGT TTT-3′) located in the intron 5 of Rad51C and reverse primer (5′ATGCATTGGCCTGGTGTT-3′) located in exon-6 of ATXN7. The expected PCR product was amplified mainly in the LS-174 T cell, human colorectal tumor (T). (PDF 91 kb)