Analysis of occult hepatitis B virus infection in liver tissue of HIV patients with chronic hepatitis C
- Resource Type
- Authors
- Giovanni Raimondo; Antonietta Cargnel; Teresa Pollicino; Teresa Santantonio; Giuseppina Raffa; Gaia Caccamo; Marco Massari; Sergio Maimone; Monica Schiavini; Giorgio Antonucci
- Source
- AIDS. 21:2171-2175
- Subject
- Adult
Male
Occult HBV infection
Hepatitis B virus
Liver DNA analysis
HBsAg
Hepatitis C virus
Immunology
HIV Infections
Genome, Viral
medicine.disease_cause
Liver disease
Orthohepadnavirus
mental disorders
Prevalence
medicine
Humans
Immunology and Allergy
Retrospective Studies
Chronic hepatitis
Occult HBV genotype
Hepatitis B Surface Antigens
biology
medicine.diagnostic_test
business.industry
Viral hepatitis coinfection
virus diseases
Hepatitis C, Chronic
Hepatitis B
biology.organism_classification
medicine.disease
Virology
digestive system diseases
Virus Latency
Infectious Diseases
Liver
Hepadnaviridae
Liver biopsy
DNA, Viral
Female
business
Viral hepatitis
psychological phenomena and processes
- Language
- ISSN
- 0269-9370
Current data on the prevalence of occult hepatitis B virus (HBV) infection in HIV-positive individuals conflict. As occult HBV infection could have an impact on the outcome of liver disease in HIV-positive patients, we investigated a large number of HIV-positive/HBV-surface-antigen (HBsAg) negative subjects with hepatitis C virus (HCV) infection by using the 'gold standard' approach for occult HBV detection--analysis of liver DNA extracts.The presence or absence of HBV DNA was determined by PCR testing of four different viral genomic regions in DNA extracts of needle liver biopsy specimens of 101 HBsAg negative individuals with HIV/HCV co-infection. HBV genotyping was performed by sequencing analysis of the preS-S gene in occult HBV isolates from 18 cases.Occult HBV infection was diagnosed in 42 of the 101 cases (41%). No clinically relevant difference was found between occult HBV-positive and -negative patients. HBV genotype D and A were detected, respectively, in 11 (61%) and 7 (39%) of 18 cases analysed.Occult HBV infection frequently occurs in HIV/HCV co-infected patients indicating the importance of performing prospective studies able to clarify its clinical impact in these patients. HBV genotype A is highly prevalent in HIV-infected subjects with occult HBV infection in a similar way to HBsAg/HIV-positive individuals.