Neuraminidase produces a decrease of adherence of slime-forming Staphylococcus aureus to gelatin-impregnated polyester fiber graft fabric: An experimental study
- Resource Type
- Authors
- Suzan Sacar; Mustafa Saçar; Gökhan Önem; Huseyin Turgut; Serhan Sakarya; Ahmet Baltalarli; İbrahim Gökşin; Vefa Ozcan
- Source
- Subject
- Staphylococcus aureus
food.ingredient
in vitro study
Prosthesis-Related Infections
bacterial colonization
bacterial count
Polyesters
Biomedical Engineering
blood vessel graft
Colony Count, Microbial
Medicine (miscellaneous)
Neuraminidase
medicine.disease_cause
Gelatin
Bacterial counts
Slime
Bacterial Adhesion
Microbiology
Biomaterials
Pathogenesis
food
medicine
Humans
controlled study
human
Incubation
Analysis of Variance
biology
Inoculation
sialidase
human cell
pathogenesis
fungi
article
graft infection
Bacterial adherence
Staphylococcal Infections
bacterium adherence
Blood Vessel Prosthesis
Polyester
surgical procedures, operative
priority journal
Biofilms
biology.protein
Prosthetic graft
Cardiology and Cardiovascular Medicine
- Language
- English
Because slime-forming microorganisms are the major causative agents of graft infections, we aimed to investigate bacterial adherence in slime-forming and nonslime-forming Staphylococcus aureus and to determine the role of neuraminidase (NANase) on adherence to gelatin-impregnated polyester fiber graft fabric. An in vitro model was developed to quantitatively measure bacterial adherence to the surface of the graft. The grafts were divided into two groups - those colonized with slime-forming S. aureus and those colonized with nonslime-forming S. aureus. The grafts were put into sterile tubes and human plasma was instilled and incubated at 37°C to perform fibrin deposition on the grafts. After 48 h of incubation, grafts were drained and inoculated with slime-forming or nonslime-forming S. aureus in triptic soy broth in the presence or absence of NANase. Following 36 h of incubation at 36°C, grafts were vortexed and cultured to perform a colony count. Bacterial counts were expressed as total colony-forming units per square centimeter of graft. Slime-forming S. aureus had greater affinity with the graft compared with nonslime-forming S. aureus (P < 0.05). The adherence of slime-forming S. aureus was impaired by NANase treatment (P < 0.001) but NANase treatment of nonslime-forming S. aureus did not change the adherence to the graft (P > 0.05). These results show that slime plays an important role in the pathogenesis of vascular graft infection. Adherence of slime-forming S. aureus can be decreased by NANase treatment. This may have implications for the development of neuraminidase-embedded vascular grafts to diminish biomaterial-related infections. © 2007 The Japanese Society for Artificial Organs.