An apolipoprotein E mimetic peptide with activities against multidrug-resistant bacteria and immunomodulatory effects
- Resource Type
- Authors
- Chuan-qing, Wang; Chang-sheng, Yang; Yi, Yang; Fu, Pan; Lei-yan, He; Ai-min, Wang
- Source
- Journal of peptide science : an official publication of the European Peptide Society. 19(12)
- Subject
- Acinetobacter baumannii
Lipopolysaccharides
Interleukin-6
Tumor Necrosis Factor-alpha
Molecular Mimicry
Microbial Sensitivity Tests
Protein Structure, Secondary
Anti-Bacterial Agents
Cell Line
Interleukin-10
Apolipoproteins E
Drug Resistance, Multiple, Bacterial
Humans
Immunologic Factors
Peptides
- Language
- ISSN
- 1099-1387
Apolipoprotein E (apoE) mimetic peptides derived from the low-density lipoprotein receptor-binding region of apoE with both activities against multidrug-resistant bacteria and immunomodulatory effects have not previously been reported. We identified an apoE mimetic peptide analogue of the receptor-binding region of apoE (abbreviated as apoE23) with the sequence of LRKLRKRLVRLASHLRKLRKRLL, which exhibited high antibacterial effects. The minimal inhibitory concentration of apoE23 against multidrug-resistant Acinetobacter baumannii was 6 µg/ml. The antimicrobial activity of apoE23 depended on its amphipathic α-helical conformation. Moreover, apoE23 downregulated the expression of tumour necrosis factor-α, interleukin-6 and interleukin-10 in lipopolysaccharide-induced THP-1 cells. ApoE23 exhibits potential in future clinical applications.