Cytoplasmic Localization of Lrh-1 Down-Regulates Ovarian Follicular cyp19a1a Expression in a Teleost, the Orange-Spotted Grouper Epinephelus coioides1
- Resource Type
- Authors
- Weimin Zhang; Lihong Zhang; Huijie Lu; Qiongyou Liu; Shen Zhang
- Source
- Biology of Reproduction. 91
- Subject
- endocrine system
medicine.medical_specialty
Orange-spotted grouper
biology
Liver receptor homolog-1
Cell Biology
General Medicine
Epinephelus
biology.organism_classification
Cell biology
Endocrinology
Reproductive Medicine
Cytoplasm
Internal medicine
Follicular phase
medicine
Grouper
Vitellogenesis
Receptor
- Language
- ISSN
- 1529-7268
0006-3363
Liver receptor homolog-1 (LRH-1) is a conserved member of the NR5A subfamily in vertebrates and a potential regulator of estrogen synthesis in the ovarian granulosa cells. An Lrh-1 homologue was obtained from the orange-spotted grouper Epinephelus coioides that contains the conserved structural features of NR5A and is phylogenetically closely related to NR5A2. The expression of the orange-spotted grouper Lrh-1 is tissue-specific with relatively higher levels in the liver and ovary. The immunoreactive signals for Lrh-1 and Cyp19a1a were present in the ovarian follicular cells and germ cells. In the ovarian follicular cells, Lrh-1 was present both in the nucleus and cytoplasm, and colocalized with Cyp19a1a. The expression levels of both increased during vitellogenesis whereas only Cyp19a1a dramatically decreased toward maturation when Lrh-1 was localized almost exclusively to the cytoplasm of the follicular cells. The orange-spotted grouper Lrh-1 could up-regulate cyp19a1a transcription in vitro vi...