Identification of β-Carotene Oxidation Products Produced by Bleaching Clay Using UPLC-ESI-MS/MS
- Resource Type
- Authors
- Jeroen S. van Duijneveldt; Paul J. Gates; Andrew J. Hambly
- Source
- Hambly, A J, Van Duijneveldt, J S & Gates, P J 2021, ' Identification of β-Carotene Oxidation Products Produced by Bleaching Clay Using UPLC-ESI-MS/MS ', Food Chemistry, vol. 353, 129455 . https://doi.org/10.1016/j.foodchem.2021.129455
- Subject
- Spectrometry, Mass, Electrospray Ionization
Canthaxanthin
medicine.medical_treatment
β-Carotene
Oxygen Isotopes
01 natural sciences
High-performance liquid chromatography
UPLC-MS
Analytical Chemistry
2-Propanol
chemistry.chemical_compound
0404 agricultural biotechnology
Adsorption
Tandem Mass Spectrometry
Acid-activated clay
Oxidation
medicine
Plant Oils
Food-Processing Industry
Carotenoid
chemistry.chemical_classification
Biological pigment
Chromatography
Plant Extracts
010401 analytical chemistry
Carotene
Water
04 agricultural and veterinary sciences
General Medicine
beta Carotene
040401 food science
Carotenoids
0104 chemical sciences
Vegetable oil
chemistry
Solvents
Clay
Surface water
Oxidation-Reduction
Food Science
Chromatography, Liquid
- Language
- English
The removal of plant pigments such as β-carotene is an aspect of vegetable oil processing often desired by the food and pharmaceutical industries. Adsorption of β-carotene to acid-activated clay (AAC) is a well-established method for purification. Despite this, the removal mechanism of β-carotene is not well understood. UPLC-MS/MS analysis of surface compounds extracted from β-carotene-AAC (BC-AAC) complexes show that AAC acts as an oxidiser. Oxidation products detected included canthaxanthin and 3',4'-didehydro-β-caroten-4-one. AAC had surface water exchanged with an 18O labelled water and was then exposed to β-carotene. Carotenoids labelled with 18O were produced from this reaction, suggesting surface water is necessary for β-carotene removal.