Association of Myosin Va and Schwann cells-derived RNA in mammal myelinated axons, analyzed by immunocytochemistry and confocal FRET microscopy
- Resource Type
- Authors
- Horst Wallrabe; Andrés Di Paolo; Alejandra Kun; José R. Sotelo-Silveira; Lucía Canclini; José R. Sotelo; Aldo Calliari
- Source
- Methods. 66:153-161
- Subject
- Confocal
Myosin Type V
Immunocytochemistry
RNA transport
Schwann cell
Biology
Ribosome
RNA Transport
General Biochemistry, Genetics and Molecular Biology
Ranvier's Nodes
Myosin
Fluorescence Resonance Energy Transfer
medicine
Animals
Peripheral Nerves
RNA, Messenger
Axon
Molecular Biology
Microscopy, Confocal
Myosin Heavy Chains
RNA
Immunohistochemistry
Axons
Rats
Cell biology
medicine.anatomical_structure
nervous system
Schwann Cells
- Language
- ISSN
- 1046-2023
Evidence from multiple sources supports the hypothesis that Schwann cells in the peripheral nervous system transfer messenger RNA and ribosomes to the axons they ensheath. Several technical and methodological difficulties exist for investigators to unravel this process in myelinated axons - a complex two-cell unit. We present an experimental design to demonstrate that newly synthesized RNA is transferred from Schwann cells to axons in association with Myosin Va. The use of quantitative confocal FRET microscopy to track newly-synthesized RNA and determine the molecular association with Myosin Va, is described in detail.