Factors affecting the generation of mast cells from multipotential colony-forming cells
- Resource Type
- Authors
- Gregory R. Johnson; C. L. Li
- Source
- Journal of cellular physiology. 121(1)
- Subject
- Physiology
Cellular differentiation
Clinical Biochemistry
Spleen
Mice, Inbred Strains
Biology
Mice
Bone Marrow
medicine
Animals
Mast Cells
Progenitor cell
Cells, Cultured
fungi
Cell Cycle
Cell Differentiation
Cell Biology
Mast cell
Hematopoietic Stem Cells
Molecular biology
Hematopoiesis
Haematopoiesis
medicine.anatomical_structure
Pokeweed Mitogens
Cell culture
Immunology
Bone marrow
Clone (B-cell biology)
- Language
- ISSN
- 0021-9541
The cells responsible for the long-term in vitro generation of murine mast cells have been examined. Sequential analysis of all colony types obtained from cultures of spleen or bone marrow cells showed that only colonies derived from multipotential cells (mixed-erythroid colonies) or mast cell progenitors, contained cells responsible for mast cell generation in liquid cultures. Primary colony growth and subsequent maintenance of mast cells in liquid cultures was dependent upon pokeweed mitogen-stimulated spleen cell-conditioned medium (SCM). Mixed-erythroid colonies from 14-day cultures of spleen cells had the greatest capacity for mast cell generation. Analysis by clone splitting and transfer to high (20%) and low (2.5%) concentrations of SCM showed that the concentration of SCM used in either the primary colony culture or subsequent liquid culture phase altered both the proliferative capacity of the mast cells generated and the frequency of mast cell progenitors within individual mixed-erythroid colonies. Thus, mixed-erythroid colonies stimulated with 2.5% SCM contained the highest proportion of mast cell progenitors (34% of colonies) and when stimulated with 20% SCM, approximately fourfold higher numbers of mast cells were produced at weekly intervals from liquid cultures maintained in 2.5% SCM compared to parallel liquid cultures containing 20% SCM. These studies confirm the hemopoietic origin of mast cells and demonstrate that a factor(s) in SCM is able to modulate their proliferative potential.