Growing evidences of the function of particular autophagy receptors indicate that most of them are often working together in the same selective autophagy pathway, such as aggrephagy, xenophagy and mitophagy. Several mitophagy receptors have been described such as scAtg32, BNIP3 and Bnip3L/Nix that interact with Atg8/LC3/GABARAP family proteins in LIR-dependent manner and recruit autophagy machinery to damaged mitochondria. This research is focused on Bnip3L/Nix receptor and its regulation. Our current results show that phosphorylation and dimerization of mitophagy receptors Nix and Bnip3 are essential for the initiation of mitochondrial sequestration by autophagic machinery and are involved in mitophagy fine-tuning.