Additional file 1 of Extracellular lipid loading augments hypoxic paracrine signaling and promotes glioma angiogenesis and macrophage infiltration
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- Offer, Svenja; Menard, Julien A.; Pérez, Julio Enríquez; de Oliveira, Kelin G.; Indira Chandran, Vineesh; Johansson, Maria C.; Bång-Rudenstam, Anna; Siesjö, Peter; Ebbesson, Anna; Hedenfalk, Ingrid; Sundgren, Pia C.; Darabi, Anna; Belting, Mattias
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Lipid loading potentiates the hypoxic response in GBM cells and patient tumors. Shown is hypoxic regulation (Hypoxia vs Normoxia) (A) and further amplification of gene expression by lipid loading (Hypoxia + LDL vs Hypoxia) (B) in GBM (U-87 MG) cells. C, Heatmap of differentially expressed genes in GBM cells and patient tumors. Column 1: Upregulated genes by hypoxia in GBM cells (Hypoxia vs Normoxia). Column 2: Hypoxia-induced genes further induced by lipid loading in GBM cells (Hypoxia + LDL vs Hypoxia). Column 3: Differential expression of genes presented in Column 1 and 2 for patient Tumor CellLD+ and Tumor CellLD− (Tumor CellLD+ vs Tumor CellLD−) areas isolated by laser microdissection. Bar graph (right panel) highlights the relative expression of commonly upregulated genes in Tumor CellLD+ vs Tumor CellLD− (gray bars) and Hypoxia + LDL vs Normoxia (black bars). Data (A-C) is presented as log2 fold-change (FC). (PDF 36 kb)