Fast Volumetric Imaging Methods for Whole Brain Imaging in Larval Zebrafish
- Resource Type
- Authors
- Chunfeng Shang; Lin Cong; Jiulin Du; Kai Wang; Zhenkun Zhang; Zeguan Wang; Wei Hang; Lu Bai
- Source
- International Conference on Photonics and Imaging in Biology and Medicine.
- Subject
- Point spread function
Functional imaging
Volumetric imaging
Microscope
Neuroimaging
law
Chemistry
Temporal resolution
fungi
Zebrafish larvae
Volume rate
Biomedical engineering
law.invention
- Language
We developed a new type of light field microscope with extended field-of-view. Using this new technique, we achieved whole larval zebrafish brain imaging at near single cell resolution and at 90 Hz volume rate. We demonstrated its applications in functional imaging of neural dynamics and fast imaging of circulating blood cells in larval zebrafish brain.