Nitrite-dependent anaerobic methane oxidation (N-DAMO) is an important biological process that combines microbial nitrogen and carbon cycling and is mainly carried out by nitrite-dependent anaerobic methane-oxidizing bacteria. The discovery of this microbial process has changed the conventional view of methane oxidation and nitrogen loss. In this study, the abundance, diversity, and community structure of N-DAMO bacteria were investigated based on high-throughput sequencing and fluorescence quantitative PCR measurements. We examined environmental factors driving the variations of CH