Preparation of Magnetic Ordered Mesoporous Carbon Composite and Its Application in Direct Electrochemistry of Horseradish Peroxidase
- Resource Type
- Authors
- Yu-Xiao Hu; Pingang He; Jing Zheng; Jia-Ling Wang; Yuzhi Fang; Jing-Li Xu; Tang-Bo He Jin; Wen-Qian Zhang
- Source
- Electroanalysis. 25:2159-2165
- Subject
- Materials science
biology
Inorganic chemistry
Composite number
Electrochemistry
Horseradish peroxidase
Amperometry
Analytical Chemistry
Matrix (chemical analysis)
chemistry.chemical_compound
chemistry
Electrode
biology.protein
Hydrogen peroxide
Biosensor
Nuclear chemistry
- Language
- ISSN
- 1040-0397
A novel magnetic ordered mesoporous carbon composite was prepared. Electrochemical measurements showed that the ordered mesoporous carbon composite provided an excellent matrix for the co-adsorption of horseradish peroxidase (HRP). HRP could be separated and collected by the application of a magnetic field and its direct electron-transfer could be achieved in the solution, not on the electrode thereby preventing the degradation of the enzyme. The cyclic voltammetric experimental results of HRP indicated that HRP displayed a pair of stable peaks with a formal potential of −0.306 V in PBS. The resulting biosensor exhibited fast amperometric response to hydrogen peroxide.