Two new phenylethanoid glycosides 1 and 2 named leonoside E and leonoside F, and one new sesquiterpene glycoside ( 3 ) identified as 7α ( H )-eudesmane-4,11 (12)-diene-3-one-2β-hydroxy-13-β- d -glucopyranoside, together with seven known glycosides ( 4 – 10 ), were isolated from the aerial part of Leonurus japonicus Houtt. Their structures were elucidated on the basis of spectroscopic data and chemical evidence. When tested in in vitro assays, compounds 1 , 2 , 4, and 6 exhibited potent hepatoprotective activity against d -galactosamine-induced toxicity in HL-7702 cells at concentration of 1 × 10 −5 M.