[Research for locating the mitochondrial DNA deletions of aged guinea-pigs]
- Resource Type
- Authors
- Xuemei, Wei; Yuan, Yang; Chuangyu, Liang; Zhong, Zheng; Yan, Zheng
- Source
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition. 34(4)
- Subject
- Male
Aging
Guinea Pigs
Animals
Female
Sequence Analysis, DNA
Cochlear Nerve
DNA, Mitochondrial
Organ of Corti
Polymerase Chain Reaction
Sequence Deletion
- Language
- ISSN
- 1672-173X
To locate the mtDNA deletions of aged guinea-pigs.By reference to the common law of mtDNA deletions in humans and rats, the possible locations of mtDNA deletions in guinea-pigs were examined by PCR and DNA sequencing. The cochlear Cortis, auditory nerve and brain tissues were harvested and the total DNA was extracted.It was discovered for the first time that there are 4568 bps deletions of mtDNA between nt8636-nt13203 in guinea-pigs. The mtDNA deletion incidence of the aged group was significantly higher than that of the young control group (P0.05).There exist 4568 bps deletions between CO III and ND5 genes of mtDNA in aged guinea-pigs; mtDNA4568 deletions of guinea-pig possibly contribute to aging.