A portable instrument-free detection method for lipopolysaccharide (LPS) analysis was developed based on dual-emission ratiometric fluorescence sensing system. Herein, red-emitting Au nanoclusters (Au NCs) were as reference probe, while blue-emitting fluorescent silica quantum dots (Si QDs) were as response probe. Additionally, the aptamer of LPS was covalently grafted to the surface of Si QDs in order to specific recognize the LPS. According to the changes of fluorescence intensityratio (FL ratio, I