Indirect Suppression of Pulsatile LH Secretion by CRH Neurons in the Female Mouse
- Resource Type
- Authors
- Xinhuai Liu; Allan E. Herbison; Robert Porteous; Isaiah Cheong; Siew Hoong Yip; Sabine Hessler
- Source
- Endocrinology. 162
- Subject
- Male
endocrine system
medicine.medical_specialty
Corticotropin-Releasing Hormone
Down-Regulation
Mice, Transgenic
Optogenetics
Inhibitory postsynaptic potential
Synaptic Transmission
Gonadotropin-Releasing Hormone
Mice
Endocrinology
Slice preparation
Kisspeptin
Arcuate nucleus
Internal medicine
medicine
Animals
Neurons
GnRH Neuron
Secretory Pathway
Chemistry
Arcuate Nucleus of Hypothalamus
Luteinizing Hormone
Mice, Inbred C57BL
medicine.anatomical_structure
nervous system
Pulsatile Flow
Female
Neuron
Luteinizing hormone
hormones, hormone substitutes, and hormone antagonists
- Language
- ISSN
- 1945-7170
0013-7227
Acute stress is a potent suppressor of pulsatile luteinizing hormone (LH) secretion, but the mechanisms through which corticotrophin-releasing hormone (CRH) neurons inhibit gonadotropin-releasing hormone (GnRH) release remain unclear. The activation of paraventricular nucleus (PVN) CRH neurons with Cre-dependent hM3Dq in Crh-Cre female mice resulted in the robust suppression of pulsatile LH secretion. Channelrhodopsin (ChR2)-assisted circuit mapping revealed that PVN CRH neuron projections existed around kisspeptin neurons in the arcuate nucleus (ARN) although many more fibers made close appositions with GnRH neuron distal dendrons in the ventral ARN. Acutely prepared brain slice electrophysiology experiments in GnRH- green fluorescent protein (GFP) mice showed a dose-dependent (30 and 300 nM CRH) activation of firing in ~20% of GnRH neurons in both intact diestrus and ovariectomized mice with inhibitory effects being uncommon (