In vitro migratory capacity of CD34(+) cells is related to hematopoietic recovery after autologous stem cell transplantation
- Resource Type
- Authors
- Hans van der Lelie; Marisha L. K. Kooi; Carlijn Voermans; Sjoerd Rodenhuis; W. R. Gerritsen; C. Ellen van der Schoot
- Source
- Blood, 97(3), 799-804. American Society of Hematology
- Subject
- Adult
Receptors, CXCR4
Adolescent
Immunology
CD34
Antigens, CD34
Biology
Biochemistry
Transplantation, Autologous
Andrology
Autologous stem-cell transplantation
In vivo
Neoplasms
Humans
Stromal cell-derived factor 1
Child
Aged
Chemotaxis
Stem Cells
Hematopoietic Stem Cell Transplantation
Cell Biology
Hematology
Middle Aged
In vitro
Chemokine CXCL12
Haematopoiesis
Child, Preschool
biology.protein
sense organs
Stem cell
Chemokines, CXC
Homing (hematopoietic)
- Language
- ISSN
- 0006-4971
To investigate whether the migratory ability of peripheral blood-derived CD34+ cells of patients undergoing autologous peripheral blood stem cell transplantation is related to the homing efficiency of these cells, the migration in vitro of these cells was determined and correlated with in vivo hematopoietic recovery. Large inter-individual differences of the in vitro migratory ability of the CD34+ cells were observed, ranging from 1.1% to 16.4% for spontaneous migration and 6.2% to 40.8% for SDF-1–induced (100 ng/mL) migration. Significantly faster hematologic recovery was observed in those patients who received transplanted CD34+cells that showed high spontaneous and SDF-1–induced migration in vitro (P